Double muscling in cattle due to mutations in the myostatin gene

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Myostatin Mutations Cause Double Muscling in Cattle

The differences between humans and farm animals are obvious, from body structure to diet to reproduction. Unlike humans, swine have litters of young; cattle are ruminants; and sheep are woolly. Despite such significant differences, growth and development of mammalian species is very similar. The biological controls that govern the establishment of body axes, induction and morphogenesis of tissu...

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Double muscling in cattle due to mutations in the myostatin gene.

Myostatin (GDF-8) is a member of the transforming growth factor beta superfamily of secreted growth and differentiation factors that is essential for proper regulation of skeletal muscle mass in mice. Here we report the myostatin sequences of nine other vertebrate species and the identification of mutations in the coding sequence of bovine myostatin in two breeds of double-muscled cattle, Belgi...

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Convenient genotyping of six myostatin mutations causing double-muscling in cattle using a multiplex oligonucleotide ligation assay.

We herein describe a procedure that allows for simultaneous genotyping of six loss-of-function mutations in the bovine myostatin gene associated with the double-muscling phenotype. The proposed method relies on a multiplex oligonucleotide ligation assay and detection of the fluorescently labelled products using automatic sequencers.

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Double Muscling in Cattle: Genes, Husbandry, Carcasses and Meat

Molecular biology has enabled the identification of the mechanisms whereby inactive myostatin increases skeletal muscle growth in double-muscled (DM) animals. Myostatin is a secreted growth differentiation factor belonging to the transforming growth factor-β superfamily. Mutations make the myostatin gene inactive, resulting in muscle hypertrophy. The relationship between the different character...

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Mutations in myostatin (GDF8) in double-muscled Belgian Blue and Piedmontese cattle.

A visibly distinct muscular hypertrophy (mh), commonly known as double muscling, occurs with high frequency in the Belgian Blue and Piedmontese cattle breeds. The autosomal recessive mh locus causing double-muscling condition in these cattle maps to bovine chromosome 2 within the same interval as myostatin, a member of the TGF-beta superfamily of genes. Because targeted disruption of myostatin ...

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ژورنال

عنوان ژورنال: Proceedings of the National Academy of Sciences

سال: 1997

ISSN: 0027-8424,1091-6490

DOI: 10.1073/pnas.94.23.12457